Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BAHCC1

Cell type

Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
Jurkat_Flag_BAHCC1_WT_Flag_ChIP
cell type
Jurkat cells
treatment condition
Flag-tagged wildtype BAHCC1
chip antibody
Mouse anti-Flag tag (M2) (Sigma F1804)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes isolated with specific antibodies against Flag tag (M2; Sigma cat # F1804), anti-acetyl-Histone H3 (H3Ac; Millipore cat# 06-599) or H3K27me3 (Abcam cat # ab6002). Libraries were prepared by High-Throughput Sequencing Facility (HTSF) at UNC at Chapel Hill.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
47007014
Reads aligned (%)
96.0
Duplicates removed (%)
17.8
Number of peaks
44587 (qval < 1E-05)

hg19

Number of total reads
47007014
Reads aligned (%)
95.5
Duplicates removed (%)
18.6
Number of peaks
48641 (qval < 1E-05)

Base call quality data from DBCLS SRA