GSM4586028: Jurkat Flag BAHCC1 WT rep#1 Flag ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BAHCC1
Cell type
Cell type Class
Blood
Cell type
Jurkat
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
Jurkat_Flag_BAHCC1_WT_Flag_ChIP
cell type
Jurkat cells
treatment condition
Flag-tagged wildtype BAHCC1
chip antibody
Mouse anti-Flag tag (M2) (Sigma F1804)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes isolated with specific antibodies against Flag tag (M2; Sigma cat # F1804), anti-acetyl-Histone H3 (H3Ac; Millipore cat# 06-599) or H3K27me3 (Abcam cat # ab6002). Libraries were prepared by High-Throughput Sequencing Facility (HTSF) at UNC at Chapel Hill.